Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Max

Cell type

Cell type Class
Blood
Cell type
RAW 264.7
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
Bone marrow
cell line
Raw264.7
cell type
Monocyte-derived cell line
treatment
RANKL+Ibet
time point
4 hours
chip antibody
Max

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The ChIP DNA was purified with AMPure beads (Beckman). ChIP and input DNA libraries were prepared using the NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (NEB) following manufacturer's protocols. The quantity was determined using the Qubit High Sensitivity DNA kit (Life Technologies) and library size was determined using the Bioanalyser High Sensitivity DNA kit (Agilent). Libraries were quantified using the Universal Library Quantification Kit for Illumina (Kapa Biosystems) and run on AB StepOne Plus Real-Time PCR (Applied Biosystems). Libraries were diluted to 2nM and sequenced at the MRC Imperial facility using the Illumina HiSeq 2500 platform to obtain single-end 50bp reads.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
64036955
Reads aligned (%)
94.3
Duplicates removed (%)
11.5
Number of peaks
10188 (qval < 1E-05)

mm9

Number of total reads
64036955
Reads aligned (%)
94.2
Duplicates removed (%)
11.5
Number of peaks
10305 (qval < 1E-05)

Base call quality data from DBCLS SRA